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h2dcf da staining  (MedChemExpress)


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    MedChemExpress h2dcf da staining
    H2dcf Da Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h2dcf+da+staining/pmc12433490-157-1-27?v=MedChemExpress
    Average 99 stars, based on 1036 article reviews
    h2dcf da staining - by Bioz Stars, 2026-08
    99/100 stars

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    Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
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    Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
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    Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
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    Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye H2DCF-DA. Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)

    Journal: Plant cell reports

    Article Title: Myosin XI coordinates ABA-induced stomatal closure via microtubule stability and ROS synthesis in drought-stressed Arabidopsis.

    doi: 10.1007/s00299-025-03538-2

    Figure Lengend Snippet: Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye H2DCF-DA. Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)

    Article Snippet: 2',7'-dichlorodihydrofluorescein diacetate (H2DCF-DA) staining assay (MedChemExpress, NJ, USA) was employed to visualize H2O2 generation in guard cells, following a previously established method (Zhou et al. 2013).

    Techniques: Labeling, Confocal Microscopy